王启要，男，1978年5月生，博士，华东理工大学生物工程学院生物反应器工程国家重点实验室教授，海洋生物学专业博士生导师，生工学院教学副院长。2013年入选上海市科技启明星人才计划，2016年入选上海市浦江人才计划和国家现代农业产业技术体系岗位科学家。2013.12-2014.12，美国哈佛医学院/霍华德·休斯医学研究院Matthew K. Waldor教授实验室访问学者。Matthew K. Waldor教授在华东理工大学的主要合作者。Microbiological Research杂志Associate Editor。

1. 海洋动物病原性细菌致病机制研究

主要针对我国海水养殖病原性细菌的典型流行菌株，采用生化分离纯化、转座子随机突变体文库以及组学（基因组学、蛋白质组学和转录组学）等策略鉴定其毒力因子，然后采用信号传导研究方法研究其受胞内外以及宿主各种信号/胁迫下的各种调控机制，描绘病原菌在致病过程以及环境适应过程中不同的毒力调控网络，从而为防治药物和疫苗的设计和组装提供靶标和相关分子器件。主要研究兴趣是迟钝爱德华氏菌和溶藻弧菌中六型分泌系统及其调控机制研究。这些研究工作与国外大学（如美国宾夕法尼亚大学、哈佛医学院、威斯康辛大学、瑞典Umeå University、英国诺丁汉大学等）相关课题组联系较为紧密。

2. 水产养殖疫苗开发相关研究

主要针对海洋养殖动物病原迟钝爱德华氏菌进行疫苗的开发工作，并且进行实际可行的多联疫苗的应用研究。这些研究与国内水产养殖相关院校、研究所和企业紧密合作。

1.Ma Y,Wang QY*, Xu WS, Liu XH, Gao XT, Zhang YX*. Stationary phase-dependent accumulation of ectoine is an efficient adaptation strategy inVibrio anguillarumagainst cold stress. Microbiological Research. 2017, accepted.

2.Gao XT, Liu Y, Liu H, Yang Z, Liu Q, Zhang YX,Wang QY*. Identification of the regulon of AphB and its essential roles in LuxR and exotoxin Asp expression in the pathogenVibrio alginolyticus. Journal of Bacteriology. 2017, doi: 10.1128/JB.00252-17.

3.Liu Y, Zhao LY, Yang MJ, Yin KY, Zhou XH, Leung KY, Liu Q, Zhang YX,Wang QY*. Transcriptomic dissection of the horizontally acquired response regulator EsrB reveals its global regulatory roles in the physiological adaptation and activation of T3SS and the cognate effector repertoire inEdwardsiella piscicidaduring infection toward turbot. Virulence. 2017. doi: 10.1080/21505594.2017.1323157.

4.Dan Gu, Huan Liu, Zhen Yang, Yuanxing Zhang,Qiyao Wang*. Chromatin immunoprecipitation sequencing technology reveals global regulatory roles of low cell density quorum-sensing regulator AphA in pathogenVibrio alginolyticus. Journal of Bacteriology 2016, 198(21):2985-2999.

5.Dan Gu, Min Guo, Minjun Yang, Yuanxing Zhang, Xiaohui Zhou*,Qiyao Wang*. A sigE-mediated temperature gauge controls a switch from LuxR-mediated virulence gene expression to thermal stress adaptation inVibrio alginolyticus. PLoS Pathogens 2016, 12(6): e1005645.

6.Lu Li,Qiyao Wang (co-first author), Hui Zhang and Xiaohui Zhou. A histidine sensor kinase detects beta-lactams and induces resistance to beta-lactam antibiotics. PNAS, 2016, doi/10.1073/pnas.1520300113

Highlighted in “Hofer U. β-lactam sensor discovered, Nature Reviews Microbiology, 2016, doi:10.1038/nrmicro.2016.27”

7.Qiyao Wang*, Yves A. Millet, Michael C. Chao, Jumpei Sasabe, Brigid M. Davis and Matthew K. Waldor*. A genome wide screen for factors controlling Vibrio cholerae virulence gene expression reveals that the phosphoenolpyruvate phosphotransferase system (PTS) modulates ToxT expression. Infect Immun, 2015, 83:3381-3395 (Q1)

8.Shuai Shao, Qiliang Lai, Qin Liu Haizhen Wu, Jingfan Xiao, Zongze Shao,Qiyao Wang*, Yuanxing Zhang. Phylogenomics characterization of a highly virulentEdwardsiellastrain ET080813encoding two distinct T3SS and three T6SS gene clusters: propose a novel species asEdwardsiella anguillarumsp. nov. Systematic and Applied Microbiology, 2015, 38: 36-47. (Q2)

9.Weizheng Yang, Lixia Wang, Lingzhi Zhang, Jiangbo Qu,Qiyao Wang*, Yuanxing Zhang. An invasive and low virulentEdwardsiella tarda esrBmutant promising as live attenuated vaccine in aquaculture. Appl Micbriol Biotechnol, 2015, 99:1765–1777 (Q1)

10.Jingfan Xiao, Tao Chen, Bin Liu, Weizheng Yang,Qiyao Wang*, Jiangbo Qu, Yuanxing Zhang.Edwardsiella tardamutant disrupted in type III secretion system and chorismic acid synthesis and cured of a plasmid as a live attenuated vaccine in turbot. Fish & Shellfish Immunology, 2013, 35: 632-641. (Q1)

11.Yuanzhi Lv, Kaiyu Yin,Shuai Shao,Qiyao Wang*, Yuanxing Zhang.Comparative proteomic analysis reveals new components of the PhoP regulon and highlights a role for PhoP in the regulation of genes encoding the F1F0 ATP synthase inEdwardsiella tarda, Microbiology-SGM, 2013, 159: 1340-1351. (Q2)

12.Lili Sheng, Yuanzhi Lv, Qin Liu,Qiyao Wang*, Yuanxing Zhang. Connecting type VI secretion, quorum sensing, and c-di-GMP production in fish pathogenVibrio alginolyticusthrough phosphatase PppA, Veterinary Microbiology, 2013, 162: 652-662

13.Yuanzhi Lv, Junyuan Zheng, Minjun Yang,Qiyao Wang*, Yuanxing Zhang. An Edwardsiella tarda mutant lacking UDP-glucose dehydrogenase shows pleiotropic phenotypes, attenuated virulence, and potential as a vaccine candidate. Veterinary Microbiology, 2012, 160: 506-512

14.Minjun Yang, Jingfan Xiao, Haizhen Wu, Yuanzhi Lv, Huajun Zheng, Qin Liu, Yuanxing Zhang,Qiyao Wang*.Edwardsiellacomparative phylogenomics reveal the new intra/inter-species taxonomic relationships, virulence evolution and niche adaptation mechanisms. PLOS ONE. 2012, 7(5): e36987.

15.Yuanzhi Lv, Jingfan Xiao, Qin Liu, Haizhen Wu, Yuanxing Zhang,Qiyao Wang*. Systematic mutation analysis of two-component signal transduction systems reveals EsrA-EsrB and PhoP-PhoQ as the major virulence regulators inEdwardsiella tarda. Veterinary Microbiology. 2012, 157:190-199

16.Huan Liu,Qiyao Wang*, Qin Liu, Xiaodan Cao, Cunbin Shi,Yuanxing Zhang*. Roles of Hfq in the stress adaptation and virulence in fish pathogenVibrio alginolyticusand its potential application as a target for live attenuated vaccine, Applied Microbiology and Biotechnology, 2011, 91:353-364

17.Xin Wang,Qiyao Wang*, Minjun Yang, Jingfan Xiao, Qin Liu, Haizhen Wu, and Yuanxing Zhang*. QseBC controls flagellar motility, fimbrial hemagglutination and intracellular virulence in fish pathogenEdwardsiella tarda. Fish & Shellfish Immunology. 2011, 30: 944-953

18.Xin Wang,Qiyao Wang*, Jingfan Xiao, Qin Liu, Haizhen Wu, Lili Xu, Yuanxing Zhang*.Edwardsiella tardaT6SS componentevpPis regulated byesrBand iron, and plays essential roles in the invasion of fish. Fish & Shellfish Immunology, 2009, 27(3): 469-477.

19.Qiyao Wang, Minjun Yang, Jingfan Xiao, Haizhen Wu, Xin Wang, Yuanzhi Lv, Lili Xu, Huajun Zheng, Shengyue Wang, Guoping Zhao, Qin Liu, Yuanxing Zhang. Genome sequence of the versatile fish pathogenEdwardsiella tardaprovides insights into its adaptation to broad host ranges and intracellular niches. PLoS ONE, 2009, 17, 4(10): e7646.

20.2016年6月2日，国际微生物学知名期刊《PLOS Pathogens》在线发表了华东理工大学生物反应器工程国家重点实验室阿华所王启要研究组关于温度调控毒力表达的信号转导机制的研究论文

王启要,刘琴,张元兴.群体感应——细菌之间的语言.生物科学与工程(叶勤主编),北京,科学出版社, 2008, 43-67.

1.刘琴,张元兴,杨朝,王启要.细菌表面展示新系统、方法及应用. ZL 101182516, 2008.05.21

2.刘琴,张元兴,杨朝,王启要.新型细菌表面展示系统、方法及应用. ZL 101195823, 2008.06.11

3.张元兴,王鑫,王启要,刘琴.一种迟钝爱德华氏菌野生毒株的无标记基因缺失减毒突变株及其应用, ZL 200910052707.6

4.王启要,张元兴,刘欢,刘琴.一种溶藻弧菌减毒株的无标记基因缺失减毒突变株、相关制剂其应用,中国发明专利.申请号：ZL 201110266453.5

5.王启要,陈涛,王鑫,肖婧凡,刘琴,张元兴.迟钝爱德华氏菌野生毒株的无标记基因缺失减毒株、相关制剂及其应用, ZL 201010541646.2

6.刘琴，张元兴，周凌云，王启要.一种多效价活疫苗、其制备方法及应用.中国发明专利.申请号：200910054113.9

7.张元兴,王启要,刘琴,吴海珍,肖婧凡.迟钝爱德华氏菌天然弱毒株及其应用.中国发明专利.申请号：201210240313.5

8.王启要,刘琴,张元兴,王亚敏,吴海珍,肖婧凡.迟钝爱德华氏菌突变株及其应用, PCT国际专利: PCT/CN2012/076961

9.王启要,王亚敏,刘琴,吴海珍,肖婧凡,张元兴.鱼用多效价活疫苗及其应用,中国发明专利.申请号：201210492313.4

10.刘琴,张元兴,郑雁,王启要.一种抗鳗弧菌和迟钝爱德华氏菌的多效价活疫苗、相关表达载体及应用.中国发明专利.专利号：201010600524.6

11.肖婧凡,张元兴,刘和松,王启要,刘琴.迟钝爱德华氏菌快速检测试纸条及快速检测方法与应用.中国发明专利.申请号：201010568826.X

12.吴海珍,张萌,李小勇,沈斌兵,王启要,张元兴.迟钝爱德华氏菌免疫保护性抗原、相关表达载体、疫苗和应用.中国发明专利.专利号：ZL201110095826.7