蔡尚，1979年生，原籍湖北浠水，干细胞生物学家，西湖大学生命科学学院研究员，博士生导师，干细胞与癌症实验室负责人。

1999年考入北京大学生命科学院生物科学专业，2003年获得学士学位。2004－2009年在印第安纳大学生物化学系攻读博士学位，从事细胞分裂过程中纺锤体组装与染色体排列的分子机制的研究。2010-2014年进入斯坦福大学干细胞与再生医学研究所进行博士后研究工作，主要研究成体干细胞及癌症干细胞的自我更新机制及分化途径。现为西湖大学生命科学学院特聘研究员。

蔡尚博士的研究主要以乳腺为模式器官，探讨乳腺干细胞在青春期、孕期、哺乳期对乳腺特定结构功能的维持，以及乳腺癌干细胞在癌症的发生、发展、抗药性、复发及转移过程中的重要作用。通过对乳腺干细胞群进行单细胞基因表达的分析，成功锁定了一类静息状态的长程干细胞亚群，首次鉴定了决定这一细胞亚群静息状态及自我更新能力的关键转录因子，并初步明确了作用机理。在癌症组织中，这一静息干细胞群被认为与癌症的抗药性相关。我们的研究为乳腺癌抗药细胞的鉴定分离及功能分析提供了重要的理论基础及实用工具。同时，我们的研究首次揭示了乳腺微环境中一类Gli2+细胞亚群的异常对乳腺疾病的致病机理，为联合性垂体激素缺乏症中的乳腺迟育问题开辟了新的治疗途径。

未来实验室将围绕干细胞的再生、谱系分化以及疾病状态下的功能异常从细胞生物学、发育生物学、遗传学、组学及癌症生物学等多方面展开全方位研究。集中进行（但不限定于）以下几个方面：

1. 乳腺细胞的谱系关系及不同发育时期的动态变化；

2. 衰老、增生及其他疾病状态下干细胞的功能异常；

3. 癌症干细胞抗药性的产生及复发转移的机理机制。

*These authors contribute equally

1. Chen Zhao*,Shang Cai*, Kunyoo Shin, Agnes Lim, Tomer Kalisky, Michael F. Clarke, Philip A. Beachy Stromal gli2 activity coordinates a niche signaling program for mammary epithelial stem cells.Science(2017) *cofirst author

2.Shang Cai, Tomer Kalisky, Debashis Sahoo, Piero Dalerba, Shaheen S. Sikandar, Neethan A. Lobo, Maider Zabala, Weiguo Feng, Yuan Lin, Angela Kong, Jeffrey Yu, Flora Wang, Elizabeth Y. Chen, Ferenc A. Scheeren, Angera H. Kuo, Shigeo Hisamori, Linda Jacqueline van Weele, Diane Heiser, Sopheak Sim, Jessica Lam, Dalong Qian, Stephen Quake and Michael F. Clarke A quiescent Bcl11b high stem cell population is required for maintenance of the mammary gland. Volume 20, Issue 2, p247–260.e5,Cell Stem Cell（2016）

3. Scheeren FA, Kuo AH, van Weele LJ,Cai S, Glykofridis I, Sikandar SS, Zabala M, Qian D, Lam JS, Johnston D, Volkmer JP, Sahoo D, van de Rijn M, Dirbas FM, Somlo G, Kalisky T, Rothenberg ME, Quake SR, Clarke MF A cell-intrinsic role for TLR2-MYD88 in intestinal and breast epithelia and oncogenesis.Nat. Cell Biol.(2014) 16, 1238-48

4. Isobe T, Hisamori S, Hogan DJ, Zabala M, Hendrickson DG, Dalerba P,Cai S, Scheeren F, Kuo AH, Sikandar SS, Lam JS, Qian D, Dirbas FM, Somlo G, Lao K, Brown PO, Clarke MF, Shimono Y miR-142 regulates the tumorigenicity of human breast cancer stem cells through the canonical WNT signaling pathway.Elife(2014) 3

5. Feng W, Gentles A, Nair RV, Huang M, Lin Y, Lee CY,Cai S,Scheeren FA, Kuo AH, Diehn M Targeting unique metabolic properties of breast tumor initiating cells.Stem Cells(2014)

6. Stephanie C. Ems-McClung, Sarah G. Hainline, Jenna Devare, Hailing Zong,Shang Cai, Stephanie K. Lamb, Sid L. Shaw, Claire E. Walczak. Aurora B inhibits MCAK activity through a phospho-conformational switch that regulates MT association.Curr. Biol.(2013) 23, 2491-9

7.Cai S, Weaver LN, Ems-McClung SC, Walczak CE. Proper Organization of microtubule minus ends is needed for the midzone stability and cytokinesis.Curr Biol.2010 May 11;20(9):880-5.

8.Cai, S., O’Connell, C. B., Khodjakov, A. and Walczak, C.E. Chromosome congression in the absence of kinetochore fibres.Nat.Cell Biol2009 Jul; 11(7):832-8.

9. Walczak CE,Cai S, Khodjakov A. Mechanisms of chromosome behaviour during mitosis.Nat Rev Mol Cell Biol.2010 Feb;11(2):91-102.

10.Cai. S.,Weaver, L.N., Ems-McClung, S.C. and Walczak, C.E. Kinesin-14 family proteins HSET/XCTK2 control spindle length by cross-linking and sliding microtubules. Mol. Biol. Cell.2009 Mar;20(5):1348-59. PMID: 19116309.

11.Cai S, Walczak CE. The road less travelled to the spindle equator.Cell Cycle. 2009 Dec;8(23):3791-3.

12.Cai, S., and Walczak, C.E. (2008) Kinetochore attachment: how the Hec can a cell do it?Curr. Biol. 18(23):1093-1096.

13. Ma, Y.,Cai, S., Lv, Q., Lv, X., Jiang, Q., Zhou, J. and Zhang, C. Inhibition of protein deacetylation by trichostatin A impairs microtubule-kinetochore attachment. Cell Mol. Life Sci. 2008; 65(19): 3100-3109.

14. Ma, Y.,Cai, S., Lv, Q., Jiang, Q., Zhang, Q, Sodmergen, Zhai, Z. and Zhang, C. Lamin B receptor plays a role in stimulating nuclear envelope production and targeting membrane vesicles to chromatin during nuclear envelope assembly through direct interaction with importin beta.J. Cell Sci.2007; 120(3):520-530.

15. Chen, Z.,Cai, S.,Jiang, Q., Zhang, C. & Tang, X. Roles for microtubule and microfilament cytoskeletons in animal cell cytokinesis.Chinese Science Bulletin, 2005; 50(3):229-235.